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Test Code HABRHD ABO Group, Rh Type and Direct Antiglobulin Test (DAT)

Collection Container

Lavender (EDTA)

Specimen Volume

4 mL

Minimum Specimen Volume

4 mL

Special Handling Instructions

Positive identification of the patient. When Applicable, compare the hospital wristband with the patient information on the request form. Label the tubes with the patient’s full name, unique identification number (eg, medical record number, typanex number), date, time, and initials of collector (usually two sets of initials for patients to be transfused)

Reasons for Rejection

Specimen improperly labeled; specimen grossly hemolyzed

Methodology

Hemagglutination (HA)

Reference Ranges

Report includes interpretation as appropriate

Day(s) and Time(s) Performed

Daily, 24 hours

Additional Information

Detects presence/absence of the A and B antigens on the red blood cells and the corresponding antibodies in the plasma. In some patients (eg newborns, elderly, or immunocomprimised patients), the expected ABO antobodies may be weak or missing. The test may be limited in its ability to detect some subgrops of type A patients. A direct antiglobulin test includes testing with a polyspecific antihuman and monospecific reagents (anti-IgG and anti-C3b-C3d) except cord blood which is tested with anti-IgG antihuman serum only. Eluates from positive cells are prepared and antibody identification performed as indicated on recently transfused patients, for investigation of immune hemolytic anemia, transfusion reactions cause by red cell incompatibility, and investigation of hemolytic disease of the newborn. Agglutination of red blood cells in the presence of antihuman serum is a positive test result which indicates the presence of human IgG and/or complement (C3b and/orC3d) on the red blood cells. Methods detect IgG immunoglobulins or complement absorbed on red cells for immune hemolytic anemias caused by antibody and/or complement components being bound to patient’s red cells, transfusion reactions due to red cell incompatibility and hemolytic disease of the newborn.

CPT Code

86900 (ABO); 86901 (Rh(D)); 86880 (each direct antiglobulin test)