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Test Code VITD25 25OH Vitamin D

Collection Container

Serum gel

Specimen Required


Specimen Volume

1 mL

Minimum Specimen Volume

0.3 mL serum

Specimen Stability

Refrigerated: 5 days

Reasons for Rejection

Grossly hemolyzed samples. Plasma is not acceptable


Direct, competitive chemiluminescence assay (CLIA)

Reference Ranges

Male and Female: 20-50 ng/mL; <12 ng/mL: At risk of vitamin D
deficiency; 12-19 ng/mL: At risk of vitamin D inadequacy; 20-50
ng/mL: Sufficient in vitamin D; >50 ng/mL: Possibly harmful
vitamin D

Turnaround Time

1 – 4 days

Day(s) and Time(s) Performed

Monday – Friday

CPT Code


Units of Measure