Test Code BITEST Testosterone, Total, Bioavailable, and Free, Serum
Additional Codes
EMR Interface Order Code: 06900
Reporting Name
Testosterone, Total, Bio, Free, SUseful For
Second- or third-order test for evaluating testosterone status (eg, when abnormalities of sex hormone-binding globulin are present)
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
TTST | Testosterone, Total, S | Yes | Yes |
FRTST | Testosterone, Free, S | No | Yes |
BATS | Testosterone, Bioavailable, S | No | Yes |
Performing Laboratory

Specimen Type
Serum RedAdvisory Information
The preferred test for diagnosis of mild abnormalities of testosterone homeostasis, particularly if abnormalities in sex hormone-binding globulin (SHBG) function or levels are present, is TTBS / Testosterone, Total and Bioavailable, Serum.
Necessary Information
Patient's age and sex are required.
Specimen Required
Container/Tube: Red top (serum gel/SST are not acceptable)
Specimen Volume: 3.5 mL
Specimen Minimum Volume
2 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum Red | Refrigerated (preferred) | 14 days | |
Frozen | 60 days |
Reference Values
TESTOSTERONE, TOTAL
Males
0-5 months: 75-400 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-130 ng/dL
12-13 years: <7-800 ng/dL
14 years: <7-1,200 ng/dL
15-16 years: 100-1,200 ng/dL
17-18 years: 300-1,200 ng/dL
≥19 years: 240-950 ng/Dl
Tanner Stages*
I (prepubertal): <7-20
II: 8-66
III: 26-800
IV: 85-1,200
V (young adult): 300-950
Females
0-5 months: 20-80 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-44 ng/dL
12-16 years: <7-75 ng/dL
17-18 years: 20-75 ng/dL
≥19 years: 8-60 ng/dL
Tanner Stages*
I (prepubertal): <7-20
II: <7-47
III: 17-75
IV: 20-75
V (young adult): 12-60
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (±2) years and for girls at a median age of 10.5 (±2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. For boys, there is no definite proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (young adult) should be reached by age 18.
TESTOSTERONE, FREE
Males (adult):
20-<25 years: 5.25-20.7 ng/dL
25-<30 years: 5.05-19.8 ng/dL
30-<35 years: 4.85-19.0 ng/dL
35-<40 years: 4.65-18.1 ng/dL
40-<45 years: 4.46-17.1 ng/dL
45-<50 years: 4.26-16.4 ng/dL
50-<55 years: 4.06-15.6 ng/dL
55-<60 years: 3.87-14.7 ng/dL
60-<65 years: 3.67-13.9 ng/dL
65-<70 years: 3.47-13.0 ng/dL
70-<75 years: 3.28-12.2 ng/dL
75-<80 years: 3.08-11.3 ng/dL
80-<85 years: 2.88-10.5 ng/dL
85-<90 years: 2.69-9.61 ng/dL
90-<95 years: 2.49-8.76 ng/dL
95-100+ years: 2.29-7.91 ng/dL
Males (children):
<1 year: Term infants
1-15 days: 0.20-3.10 ng/dL*
16 days-1 year: Values decrease gradually from newborn (0.20-3.10 ng/dL) to prepubertal levels
*Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142
1-8 years: <0.04-0.11 ng/dL
9 years: <0.04-0.45 ng/dL
10 years: <0.04-1.26 ng/dL
11 years: <0.04-5.52 ng/dL
12 years: <0.04-9.28 ng/dL
13 years: <0.04-12.6 ng/dL
14 years: 0.48-15.3 ng/dL
15 years: 1.62-17.7 ng/dL
16 years: 2.93-19.5 ng/dL
17 years: 4.28-20.9 ng/dL
18 years: 5.40-21.8 ng/dL
19 years: 5.36-21.2 ng/dL
Females (adult):
20-<25 years: 0.06-1.08 ng/dL
25-<30 years: 0.06-1.06 ng/dL
30-<35 years: 0.06-1.03 ng/dL
35-<40 years: 0.06-1.00 ng/dL
40-<45 years: 0.06-0.98 ng/dL
45-<50 years: 0.06-0.95 ng/dL
50-<55 years: 0.06-0.92 ng/dL
55-<60 years: 0.06-0.90 ng/dL
60-<65 years: 0.06-0.87 ng/dL
65-<70 years: 0.06-0.84 ng/dL
70-<75 years: 0.06-0.82 ng/dL
75-<80 years: 0.06-0.79 ng/dL
80-<85 years: 0.06-0.76 ng/dL
85-<90 years: 0.06-0.73 ng/dL
90-<95 years: 0.06-0.71 ng/dL
95-100+ years: 0.06-0.68 ng/dL
Females (children):
<1 year: Term infants
1-15 days: 0.06-0.25 ng/dL*
16 days-1 year: Values decrease gradually from newborn (0.06-0.25 ng/dL) to prepubertal levels
*Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142
1-4 years: <0.04 ng/dL
5 years: <0.04-0.07 ng/dL
6 years: <0.04-0.14 ng/dL
7 years: <0.04-0.23 ng/dL
8 years: <0.04-0.34 ng/dL
9 years: <0.04-0.46 ng/dL
10 years: <0.04-0.59 ng/dL
11 years: <0.04-0.72 ng/dL
12 years: <0.04-0.84 ng/dL
13 years: <0.04-0.96 ng/dL
14 years: <0.04-1.06 ng/dL
15-18 years: <0.04-1.09 ng/dL
19 years: 0.06-1.08 ng/dL
TESTOSTERONE, BIOAVAILABLE
Males
≤19 years: not established
20-29 years: 83-257 ng/dL
30-39 years: 72-235 ng/dL
40-49 years: 61-213 ng/dL
50-59 years: 50-190 ng/dL
60-69 years: 40-168 ng/dL
≥70 years: not established
Females (non-oophorectomized)
≤19 years: not established
20-50 years (on oral estrogen): 0.8-4.0 ng/dL
20-50 years (not on oral estrogen): 0.8-10 ng/dL
>50 Years: not established
Day(s) and Time(s) Performed
Monday through Saturday; 1 p.m.
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
84402
84403
84410
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TTFB | Testosterone, Total, Bio, Free, S | 58716-2 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
82978 | Testosterone, Bioavailable, S | 2990-0 |
3631 | Testosterone, Free, S | 2991-8 |
8533 | Testosterone, Total, S | 2986-8 |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | Reject |
Method Name
FRTST: Equilibrium Dialysis
TTST: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
BATS: Differential Precipitation