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Test Code D125OH, (1,25 di-OH Vitamin D) Calcitriol 1,25(OH) Vitamin D, 1,25-Dihydroxy Vitamin D, 1,25-Dihydroxycholecalciferol, Vitamin D, 1,25-Dihydroxy

Important Note

USE:Aid in the diagnosis of primary hyperparathyroidism, hypoparathyroidism, pseudohypoparathyroidism, renal osteodystrophy, and vitamin D-resistant rickets.


This test is not the same as Vitamin D, 25-Hydroxy [081950] (vitamin D3), which must be ordered separately.

Collection Container

gel or heparin(green) or lavender top edta plasma (poured off in aliquot tube)

Specimen Required

Serum or plasma


Specimen Volume

0.5 mL

Minimum Specimen Volume

0.3 (Note: This volume does not allow for repeat testing.)

Transport Temperature

Room Temp. (Sent refridgerated)


Specimen Stability

Room Temp: 10 days

Refrigerated: 10 days

Frozen: 10 days

Freeze/thaw cycle: Stable x3


Immunochemiluminometric assay (ICMA)

Days and Times Performed

Monday through Friday

Turnaround Time

5 days

Reference Ranges

19.9 - 79.3 ppg/mL

CPT Code