Sign in →

Test Code HABRHD ABO Group, Rh Type and Direct Antiglobulin Test (DAT)

Additional Information

Detects presence/absence of the A and B antigens on the red blood cells and the corresponding antibodies in the plasma. In some patients (eg: newborns, elderly, or immunocompromised patients), the expected ABO antibodies may be weak or missing. The test may be limited in its ability to detect some subgroups of type A patients.


A Direct Antiglobulin Test (DAT) includes testing with a polyspecific antihuman and monospecific reagents (anti-IgG and anti-C3b- C3d) except cord blood which is tested with anti-IgG antihuman serum only.  Eluates from positive cells are prepared and antibody identification performed as indicated on recently transfused patients, for investigation of immune hemolytic anemia, transfusion reactions cause by red cell incompatibility, and investigation of hemolytic disease of the newborn.

Agglutination of red blood cells in the presence of antihuman serum is a positive test result which indicates the presence of human IgG and/or complement (C3b and/or C3d) on the red blood cells. Methods detect IgG immunoglobulins or complement absorbed on red cells for immune hemolytic anemias caused by antibody and/or complement components being bound to patients' red cells, transfusion reactions due to red cell incompatibility and hemolytic disease of the newborn.

Collection Container

Lavender (EDTA)

Special Handling Instructions

Positive identification of the patient. When applicable, compare the hospital wristband with the patient information on the request form. Label the tubes with the patients' full name, unique identification number (eg: medical record number, typenex number), date, time, and initials of collector (two sets of initials for patients to be transfused)

Specimen Volume

4 mL

Minimum Specimen Volume

4 mL

Reasons for Rejection

Specimen improperly labeled; specimen grossly hemolyzed


Hemagglutination (HA)

Days and Times Performed

Daily, 24 hours

Reference Ranges

Report includes interpretation as appropriate

CPT Code

86900 (ABO); 86901 (Rh(D)); 86880 (each Direct Antiglobulin Test)

EMR Interface Order Code