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Test Code VITD25 25OH Vitamin D

Collection Container

Serum gel preferred. Heparinized plasma and EDTA plasma also accepted

Specimen Required


Specimen Volume

1 mL

Minimum Specimen Volume

0.3 mL serum

Specimen Stability

Room Temperature: 8 hours, Refrigerated: 4 days, Frozen: 24 weeks


Electrochemiluminescence immunoassay (ECLIA)

Days and Times Performed

Monday - Sunday

Turnaround Time

24 hours

Reference Ranges

Male and Female: 20 - 50 ng/mL

     <12 ng/mL: At risk of Vitamin D deficiency

     12 - 19 ng/mL: At risk of Vitamin D inadequacy

     20 - 50 ng/mL: Sufficient in Vitamin D

     >50 ng/mL: Possibly harmful Vitamin D

Units of Measure


CPT Code


EMR Interface Order Code